1. Senju Mito: Mito, the wife of the first Hokage and the first Jinchuriki of the Nine-Tails, possessed the powerful sealing Jutsu of the Senju clan. The clan's members, including Mito, have strong vitality and large chakra reserves. Tsunade may have learned the Hundred Healings Technique from her grandmother, as both the Senju and Uzumaki clans have chakra reserves suitable for this technique.

2. Shizune: Shizune is one of Tsunade's disciples but has yet to fully master the Hundred Healings Technique.

However, continuous chakra accumulation on her forehead suggests she will achieve mastery soon.

3. Sakura: Sakura, another disciple of Tsunade, mastered the Hundred Healings Technique in half the time it took Shizune. She played a vital role in the Fourth Great Ninja War, using her powerful healing abilities to assist Naruto and Sasuke.

4. Tsunade: The Fifth Hokage is the most skilled user of the Hundred Healings Technique.

Her Senju heritage allowed her to unlock the seals multiple times with ease.

5. First Hokage (Hashirama Senju): Known as the "God of Shinobi," he had exceptional abilities and was the only one who could match Uchiha Madara. His mastery of Wood Release and the regenerative powers of the Senju clan made the Hundred Healings Technique a passive ability for him.